Journal: Environmental science & technology

Article Title: Adaptable Plasmonic Membrane Sensors for Fast and Reliable Detection of Trace Low-Micrometer Microplastics in Lake Water.

doi: 10.1021/acs.est.4c06503

Figure Lengend Snippet: Figure 2. Raman spectra and maps of 1.5 μm PS LMMPs on unmodified 0.4 μm PCTE membrane and 0.4 μm PCTE membrane sensors. Raman spectra of 1.5 μm PS on (a) 0.4 μm PCTE membrane and (f) 0.4 μm PCTE membrane sensors. Individual Raman spectra of 10 particles were measured and averaged. The averaged Raman spectra (darker green line), along with the upper and lower bounds of 1 SD (lighter green shade), were plotted. (b) Dark-field image of 1.5 μm PS on the 0.4 μm PCTE membrane and the corresponding Raman maps by tracking the PS Raman bands at (c) 996 cm−1, (d) 3053 cm−1, and (e) 1597 cm−1. (g) Dark-field image of 1.5 μm PS on the 0.4 μm PCTE membrane sensor and the corresponding Raman maps by tracking the PS Raman bands at (h) 996 cm−1, (i) 3053 cm−1, and (j) 1597 cm−1. The acquisition time of each Raman spectrum is 0.1 s.

Article Snippet: A scanning electron microscope (Zeiss GeminiSEM 450, Zeiss, Germany) and an optical microscope equipped with a confocal Raman spectrometer (Horiba XploRA PLUS) were used to characterize the surface morphology of both gold-coated and pristine membranes by capturing and comparing images acquired at various magnification levels.

Techniques: Membrane

Journal: Environmental science & technology

Article Title: Adaptable Plasmonic Membrane Sensors for Fast and Reliable Detection of Trace Low-Micrometer Microplastics in Lake Water.

doi: 10.1021/acs.est.4c06503

Figure Lengend Snippet: Figure 3. SERS spectra of 4-MBA on PCTE membrane sensors with the change of gold layer thickness and membrane pore size. (a) SERS spectra of 4-MBA on 0.4 μm PCTE membrane sensors with different gold layer thicknesses. The thickness of the gold layer coated on the membrane sensor is presented by the coating time ranging from 0 to 40 s. (b) SERS spectra of 4-MBA on PCTE membrane sensors with different pore sizes and the same gold layer thickness (sputter coating time: 30 s). (c) Variation of the SERS intensity of the 4-MBA Raman band at 1065 cm−1 as a function of the gold layer thickness. (d) Variation of the SERS intensity of the 4-MBA Raman band as a function membrane pore size. In (c) and (d), the intensities of the 4- MBA Raman band at 1065 cm−1 were normalized with the intensities of the PCTE membrane Raman band at 876 cm−1. The two most intense and commonly tracked Raman bands of 4-MBA at 1065 and 1575 cm−1 were labeled with red dashed lines.

Article Snippet: A scanning electron microscope (Zeiss GeminiSEM 450, Zeiss, Germany) and an optical microscope equipped with a confocal Raman spectrometer (Horiba XploRA PLUS) were used to characterize the surface morphology of both gold-coated and pristine membranes by capturing and comparing images acquired at various magnification levels.

Techniques: Membrane, Pore Size, Labeling

Journal: Environmental science & technology

Article Title: Adaptable Plasmonic Membrane Sensors for Fast and Reliable Detection of Trace Low-Micrometer Microplastics in Lake Water.

doi: 10.1021/acs.est.4c06503

Figure Lengend Snippet: Figure 4. Dark-field images and Raman maps of 1.5 μm PS LMMPs on 0.4 μm PCTE membrane sensors with lake water sampled in Summer 2023. (a) Dark-field image of 1.5 μm PS on the 0.4 μm PCTE membrane sensor spiked in filtered lake water with a concentration of 0.2 mg L−1 and (b) corresponding Raman map. (c) Dark-field image of 1.5 μm PS on the 0.4 μm PCTE membrane sensor spiked in raw lake water with a concentration of 0.2 mg L−1

Article Snippet: A scanning electron microscope (Zeiss GeminiSEM 450, Zeiss, Germany) and an optical microscope equipped with a confocal Raman spectrometer (Horiba XploRA PLUS) were used to characterize the surface morphology of both gold-coated and pristine membranes by capturing and comparing images acquired at various magnification levels.

Techniques: Membrane, Concentration Assay

Journal: Nano-micro letters

Article Title: Proof of Aerobically Autoxidized Self-Charge Concept Based on Single Catechol-Enriched Carbon Cathode Material.

doi: 10.1007/s40820-023-01283-3

Figure Lengend Snippet: Fig. 2 Structural characterization of oxygen-enriched carbon (PF) material. a Raman spectrum. b TEM, c HADF-STEM images and C, O ele- mental mapping. d High-resolution XPS spectrum of O 1s. e, f High-resolution NEXAFS spectra of C and O K-edge. g Solid-state 13C NMR spectrum

Article Snippet: Raman spectra were recorded with a Raman microscope (HORIBA Xplora) using an Ar ion laser at an excitation wavelength of 532 nm.

Techniques:

Journal: Scientific reports

Article Title: Raman spectroscopic signatures of carotenoids and polyenes enable label-free visualization of microbial distributions within pink biofilms.

doi: 10.1038/s41598-020-64737-3

Figure Lengend Snippet: Figure 1. Optical microscopy and Raman microspectroscopy measurements of a pink biofilm collected from the drain of a bathroom. (A) Optical micrograph of the pink biofilm. Scale bar, 20 μm. (B) Enlarged view of the region in which we performed a Raman imaging experiment. This region is indicated by a square in A. Scale bar, 2 μm. (C) 532-nm excited Raman spectra (raw data) measured at points a (red line) and b (blue line) shown in B. The spectra were taken with 1 mW laser power and a 0.5 s exposure time. Neither smoothing nor noise reduction was performed. (D) Comparison of the C=C stretching band in the Raman spectra measured at points a (red line) and b (blue line). The bands are normalized to the peak height.

Article Snippet: These data were recorded with a commercial upright-type Raman microscope (HORIBA XploRA Nano) equipped with a 532-nm laser.

Techniques: Microscopy, Imaging, Comparison

Journal: Scientific reports

Article Title: Raman spectroscopic signatures of carotenoids and polyenes enable label-free visualization of microbial distributions within pink biofilms.

doi: 10.1038/s41598-020-64737-3

Figure Lengend Snippet: Figure 2. Construction and comparison of the Raman images at 1128 and 1154 cm−1. (A) Baseline-corrected Raman spectrum at point b in Fig. 1B (black line) and best fit to a sum of three Lorentzian functions (thick red line). The baseline correction was done using polynomial functions (see Methods for details). (B,C) Distribution maps of the area intensities of the Lorentzian functions accounting for the 1154 cm−1 band (B) and the 1128 cm−1 band (C). The scanned area corresponds to Fig. 1B. The Raman images are displayed in rainbow pesudocolor with red representing the highest intensity and purple the lowest.

Article Snippet: These data were recorded with a commercial upright-type Raman microscope (HORIBA XploRA Nano) equipped with a 532-nm laser.

Techniques: Comparison

Journal: Scientific reports

Article Title: Raman spectroscopic signatures of carotenoids and polyenes enable label-free visualization of microbial distributions within pink biofilms.

doi: 10.1038/s41598-020-64737-3

Figure Lengend Snippet: Figure 3. MCR–ALS Analysis of the Raman hyperspectral imaging data of the pink biofilm assuming three components (k = 3). (A) Intrinsic Raman spectra of the three components (denoted 1–3). Each spectrum has been normalized so that the sum of intensities at all Raman shifts is equal to unity. The spectra are vertically offset for clarity of display. (B–D) Raman images of components 1 (B), 2 (C), and 3 (D). The Raman images are shown in pseudocolor. (E) Merged image of B–D, indicating mutually exclusive distributions of components 1 (magenta) and 2 (green). Colocalization of components 2 (green) and 3 (red) is manifested as a yellow region (indicated by an arrow).

Article Snippet: These data were recorded with a commercial upright-type Raman microscope (HORIBA XploRA Nano) equipped with a 532-nm laser.

Techniques: Imaging

Journal: Scientific reports

Article Title: Raman spectroscopic signatures of carotenoids and polyenes enable label-free visualization of microbial distributions within pink biofilms.

doi: 10.1038/s41598-020-64737-3

Figure Lengend Snippet: Figure 4. Raman spectra of microbial cells isolated from the pink biofilm sample. (A) Averaged Raman spectra (a–g) of 10 different single cells taken from the seven colonies isolated from the same pink biofilm as in Figs. 1–3. Also shown are pictures of the colonies on agar plates (scale bar, 0.5 mm). The Raman spectra were measured in R2A medium. Asterisks denote resonance Raman bands of cytochromes. The arrow highlights a shoulder adjacent to the intense cytochrome band at ~1128 cm−1. (B) Difference spectra between b and e (e minus b; red line) and between b and g (g minus b; blue line). In doing subtraction, an appropriate coefficient was multiplied to spectrum b so that the major Raman bands of proteins including cytochromes were mostly cancelled. Yet, subtraction artifacts due to inadequate cancelation of the protein Raman bands (marked with #) are seen in the difference spectrum between b and e (red line).

Article Snippet: These data were recorded with a commercial upright-type Raman microscope (HORIBA XploRA Nano) equipped with a 532-nm laser.

Techniques: Isolation

Journal: Scientific reports

Article Title: Raman spectroscopic signatures of carotenoids and polyenes enable label-free visualization of microbial distributions within pink biofilms.

doi: 10.1038/s41598-020-64737-3

Figure Lengend Snippet: Figure 5. Representative space-resolved Raman spectra measured within pink biofilms collected from a bathroom in different homes in Takarazuka (A) and Toyonaka (B). These data were recorded with a commercial upright-type Raman microscope (HORIBA XploRA Nano) equipped with a 532-nm laser. The excitation laser power at the sample point was ~4 mW, and the exposure time was 5 s. A 100×, oil-immersion objective (NA = 0.9) was used.

Article Snippet: These data were recorded with a commercial upright-type Raman microscope (HORIBA XploRA Nano) equipped with a 532-nm laser.

Techniques: Microscopy

Journal: NanoImpact

Article Title: Mapping 2D- and 3D-distributions of metal/metal oxide nanoparticles within cleared human ex vivo skin tissues

doi: 10.1016/j.impact.2020.100208

Figure Lengend Snippet: (a, b) Calculated scattering efficiency (Qsca) of TiO2 and ZnO at different diameters and multiple wavelengths, based on Lorenz-Mie theory model of particle scattering. (c, d) Enhanced darkfield images of ENM-exposed skin tissue sections overlaid with (c) TiO2 and (d) ZnO distribution map; annotated as red highlighted regions. White arrow in (d) indicates the epidermal region. (e, f) Representative scattering spectra taken from hyperspectral scans of tissue samples compared to a reference slide with TiO2 or ZnO aqueous solutions and background dermal tissue signal. (g) Darkfield-visible TiO2 ENMs in cleared skin sections; merged with brightfield image. (h) Raman scans of points in a representative cleared skin section with darkfield-visible scattering signal; compared to a reference TiO2 and blank glass slide. Black arrows in (g) represent the spots corresponding to the reported Raman spectra in (h). For these 24 h exposure experiments, only the dermal side of the biopsies was exposed to ENM suspensions.

Article Snippet: Cleared and uncleared skin sections were imaged on a darkfield hyperspectral microscope (Cytoviva) integrated with a confocal Raman microscope (Horiba XploRA PLUS) in transmission mode.

Techniques:

Journal: NanoImpact

Article Title: Mapping 2D- and 3D-distributions of metal/metal oxide nanoparticles within cleared human ex vivo skin tissues

doi: 10.1016/j.impact.2020.100208

Figure Lengend Snippet: (a-b) Darkfield images of 50 μm skin sections before and after clearing. (c-d) Brightfield images of (a) and (b). (e) Representative intensity profile of skin sections before and after clearing (6 line scans from 1 skin tissue section). (f) Darkfield signal intensity before and after clearing in the dermal and epidermal regions (total of 14 fields of view (FOV) from n = 2 sections from 1 sample). Scale bars = 200 μm. *p < 0.0005; **p < 0.0001. Error bars represent SEM.

Article Snippet: Cleared and uncleared skin sections were imaged on a darkfield hyperspectral microscope (Cytoviva) integrated with a confocal Raman microscope (Horiba XploRA PLUS) in transmission mode.

Techniques:

Journal: NanoImpact

Article Title: Mapping 2D- and 3D-distributions of metal/metal oxide nanoparticles within cleared human ex vivo skin tissues

doi: 10.1016/j.impact.2020.100208

Figure Lengend Snippet: (a-c) Whole skin tissue sections (thickness ~50 μm) were stained for DAPI and cytokeratin 14, cleared, and imaged with a confocal microscope to identify keratin- and cell-rich skin layers and appendages including the epidermis, hair follicles, sebaceous glands and sweat glands. (d-i) After confocal imaging, sections were imaged with darkfield microscopy to identify regions of increased scattering where applied ENMs permeated into the tissue. Skin samples in (a, d, g) did not have any treatment applied, while other samples were exposed to 100 μg/mL of (b, e, h) TiO2 and (c, f, i) ZnO nanoparticles for 24 hours. For these exposure experiments, only the dermal side of the biopsies was exposed to ENM suspensions.

Article Snippet: Cleared and uncleared skin sections were imaged on a darkfield hyperspectral microscope (Cytoviva) integrated with a confocal Raman microscope (Horiba XploRA PLUS) in transmission mode.

Techniques: Staining, Microscopy, Imaging

Journal: NanoImpact

Article Title: Mapping 2D- and 3D-distributions of metal/metal oxide nanoparticles within cleared human ex vivo skin tissues

doi: 10.1016/j.impact.2020.100208

Figure Lengend Snippet: (a-d) Darkfield images with (e-h) hyperspectral maps of ZnO-positive pixels in areas with high background scattering even after iDISCO-clearing. (a, e) Dermal region of an unexposed tissue is shown for reference. Scale bars = 200 μm. For these exposure experiments, only the dermal side of the biopsies was exposed to ENM suspensions.

Article Snippet: Cleared and uncleared skin sections were imaged on a darkfield hyperspectral microscope (Cytoviva) integrated with a confocal Raman microscope (Horiba XploRA PLUS) in transmission mode.

Techniques: